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| Genetic Tests: How to Interpret Results and Incorporate Them into Your Breeding Program Jerold S Bell DVM, Clinical Associate Professor of Genetics, Tufts Cummings School of Veterinary Medicine (This article was originally published in the proceedings of the AKC Canine Health Foundation 2011 National Parent Club Canine Health Conference. It is reprinted here with the written permission of Jerold S. Bell DVM) Genetic tests vary on what they are able to identify, and therefore how they can be used in managing genetic disease. Phenotypic tests: Some tests measure the phenotype, or what can be seen in the animal. This may not directly relate to the genotype, or the genes regulating the defect that you are trying to manage. Screening for cataracts, ausculting for heart murmurs, hip and elbow radiographs, thyroid profiles, urinalysis for crystals or metabolites, skin biopsy for sebaceous adenitis, and observations on behavioral traits are all tests of the phenotype. Most tests of the phenotype only identify affected individuals, and not carriers of disease liability genes. Linked-marker based tests: Some defective genes can be linked to a genetic marker, which could be tested for. Linked-marker based tests do not identify the defective gene, but a marker that lies close on the chromosome. If a crossover occurs between the marker and the defective gene during reproduction, the marker will no longer be linked to the defective gene. False positive and false negative results will occur. Due to this phenomenon, linkage test results must be compared with results from other family members to determine whether they correlate with the known genotype of relatives. Linked marker tests include those for cerebellar ataxia in Italian Spinone and primary hyperparathyroidism in Keeshond. Direct mutation based tests: Direct gene tests are specific for mutations and are a direct measurement of the genotype. They can identify affected, carrier, and normal individuals. These can be run at any age, regardless of the age of onset of the disorder. Most direct gene tests identify a mutation that is causative for a genetic disorder. However, some direct genetic tests identify a mutation that causes an increased susceptibility for genetic disease. These susceptibility alleles can be part of polygenic/complexly inherited traits, or the cause of incomplete penetrance of (assumed) simple Mendelian traits. Examples are cord1 PRA in English Springer Spaniels and Miniature Dachshunds, and degenerative myelopathy in several dog breeds. Degenerative myelopathy is considered a complexly inherited disease. An autosomal recessive susceptibility gene has been identified that is homozygous (two abnormal copies) in all DM affected dogs. However, a large proportion of individuals in these breeds are homozygous for this gene and do not become affected. They are considered “at risk”, but not genetically affected. In the Boxer, less than 0.5% of dogs develop the disease. Testing Boxers for the DM susceptibility gene shows 39% testing carrier, and 43% testing homozygous for the susceptibility gene. This test is useful in determining individual dogs with and without risk of developing DM. However, selecting against 82% of the Boxer gene pool when making breeding decisions, when the majority will not produce the disorder is detrimental to the genetic diversity of the breed. Other susceptibility genes are found to occur at a greater frequency in affected animals, but are not present in all affected animals. An example is the susceptibility gene for perianal fistula/anal furunculosis in German Shepherd Dogs. Dogs with the susceptibility haplotype (specific sequence of genes) have a 3.7X odds ratio for the disease versus those without the haplotype. Another example is the genetic test for Pug Dog Encephalitis, where dog homozygous for a susceptibility haplotype have a 12.7X odds ratio for developing the disease. Utilizing Genetic Tests We need to be knowledgeable about what genetic tests are available, and in which individuals they should be run. Dogs from breeds with an incidence of von Willebrand disease should be tested early in life, so that measures can be taken to prevent excessive hemorrhage during surgery or injury. Dogs in breeds at risk of carrying the mdr-1 drug sensitivity mutation should be tested early in life, before drug treatment. In high-risk breeds for serious genetic disease, individual animals should be genetically tested (or verified results documented for parents) before purchase. These include Boxers for arrythmogenic right ventricular cardiomyopathy, and Doberman Pinschers for dilated cardiomyopathy. We need to understand the temporal periods when genetic testing will be most accurate, and allow for intervention. Genetic testing for inherited hypothyroidism (autoimmune thyroiditis) is based on the presence of thyroglobulin autoantibodies. A dog with normal TgAA levels on two tests at least two years apart between two and six years of age is phenotypically normal. However, TgAA levels should not be measured within 2-3 months post-vaccination, as a transient iatrogenic rise can occur during this period. For most genetic diseases, we know how to either prevent their occurrence, or at least lessen the possibility of producing offspring with genetic disease. This can occur through the genotypic testing of the parents (identification of parents carrying liability genes for genetic disease), phenotypic testing of the parents (identification of parents affected with genetic disease), or pedigree analysis (identification of carrier and affected risk based on the knowledge of carrier or affected relatives). The genetic improvement of dogs will only occur through selective breeding. Inherent in this point is the acknowledgement that breeding without genetic testing is irresponsible, and unethical. Genetic testing is health quality control. It is no longer acceptable for a breeder to choose two individuals and breed them together without regard to genetic disease control. The most important goal of managing genetic disease is to avoid producing affected individuals. The secondary goal is to reduce the frequency of carriers of defective genes in the population. At the same time, recommendations should allow perpetuation of breeding lines, in order to preserve the genetic diversity of the population. With each new generation, breeders ask, “How can I continue my line and improve it?” Aside from selecting for conformation, behavior and general health, breeders must consider how they are going to reduce the incidence of whichever genetic disorders are present in their breed. There are no answers that will fit every situation. There are, however, guidelines to preserve breeding lines and genetic diversity while reducing the risk of producing animals that carry defective genes, or are affected with genetic disorders. Autosomal Recessive Disorders In the case of a simple autosomal recessive disorder for which a direct genetic test for carriers is available, the recommendation is to test breeding-quality stock, and breed normals to normals, or carriers to normal-testing individuals. This prevents affected offspring from being produced. Breeders are the custodians of their breed's past and future. "Above all, do no harm" is a primary oath of all medical professionals. Genetic tests are powerful tools, and their use can cause significant positive or negative changes to breed gene pools. Once a genetic test is developed that allows breeders to determine if an animal is a carrier of a defective gene, many owners are likely to simply eliminate carriers from breeding. Although doing so is human nature, this temptation must be overcome. If an owner would breed an individual if it tested normal for a genetic disease, then a carrier result should not change that decision. A direct genetic test should not alter WHO gets bred, only WHO THEY GET BRED TO. One defective gene that can be identified through a genetic test out of tens of thousands of genes is not a reason to stop breeding. A genetic test that should be used to help maintain breed quality and diversity should not result in limiting it. We know that most individuals carry some unfavorable recessive genes. The more genetic tests that are developed, the greater chance there is of identifying an undesirable gene in a breeding animal. History has shown that breeders can be successful in reducing breed-wide genetic disease through testing and making informed breeding choices. However, there are also examples of breeds that have actually experienced more problems as a result of unwarranted culling and restriction of their gene pools. These problems include: 1) Reducing the incidence of one disease and increasing the incidence of another by repeated use of males known to be clear of the gene that causes the first condition. 2) Creating bottlenecks and diminishing diversity by eliminating all carriers of a gene from the breeding pool, instead of breeding and replacing them. 3) Concentrating on the presence or absence of a single gene and not the quality of the whole animal. The aim is to replace the carrier breeding-animal with a normal-testing offspring that equals or exceeds it in quality. Additional carrier testing offspring should not be placed in breeding homes; as the goal is to reduce the frequency of the defective gene in the population. As each breeder tests and replaces carrier animals with normal-testing offspring, the problem for the breed as a whole diminishes, while not restricting gene pool diversity. The problem with a simple autosomal recessive disorder for which no carrier test exists is the propagation and dissemination of unapparent carriers in the gene pool. A quality individual that is found to be a carrier of a recessive gene can be retired from breeding and replaced with a quality relative or prior-born offspring. The genes of the retired individual can thus be preserved through the selected relative, but the carrier risk can be cut in half. To further limit the spread of the defective gene, the offspring should be used in only a limited number of carefully planned matings, and then should also be replaced with one or two representative offspring. The rest of the litter should be placed in non-breeding (pet) homes. With this mating scheme, you are maintaining the good genes of the line, reducing the carrier risk with each generation, and replacing, not adding to the overall carrier risk in the breeding population. Breeders must assess the carrier risk of each individual animal in their breeding program. An open health registry that is supported by the parent club makes it easier for breeders to objectively assess these matters. An example is the genetic disease control program for cerebellar abiotrophy by the Scottish Terrier Club of America http://www.stca.biz/health-registries/ca-registry. By determining the average carrier-risk for the breeding population, breeders can select matings that have a projected risk that is lower than the breed average. Relative risk assessments only take into account the identified carrier and affected individuals in the pedigree. Therefore, these estimates determine the minimum risk based on the information available. If additional affected relatives to the pedigree are diagnosed, the computed risk will rise. The relative risk pedigree calculator on the Scottish Terrier website can be used by any breed to compute carrier and affected risk for any simple autosomal recessive disorder. If a quality breeding animal is at high risk of being a carrier, the best advice is to breed to an individual that has a low risk. Using relative-risk assessment as a tool, breeders should replace higher-risk breeding animals with lower-risk offspring that are equal to or better than their parents in quality. A negative aspect of pedigree analysis is that it selects against families, regardless of an individual’s normal or carrier status. On the other hand, it allows for the objective risk assessment and continuation of lines that might otherwise be abandoned due to high carrier-risk. Autosomal Dominant Disorders Autosomal dominant genetic disorders are usually easy to manage. Each affected animal has at least one affected parent, but it can be expected that half of the offspring of an affected animal will be free of the defective gene. With disorders that cause death or discomfort, the recommendation is to not breed affected animals. To produce the next generation of a line; a normal full sibling to an affected animal, a normal close relative, or the parent that is normal can be used. If the defective gene is at a high frequency in the gene pool, eliminating all affected breeding animals in one generation may have a significant negative impact on genetic diversity. When a high frequency autosomal dominant disorder is first identified, some quality, affected animals may have to be bred, and replaced with quality, normal testing offspring. However, once a few generations have gone by and breeders have had the opportunity to replace affected with normal individuals, the continued breeding of affected animals is not ethical. A problem with some autosomal dominant disorders is incomplete penetrance; where some animals with the defective gene may not show the disorder. Roughly half their offspring, however, may be affected. If a genetic test is available, this is not a problem. Otherwise, pedigree analysis and relative-risk assessment can identify which animals are at risk of carrying incompletely penetrant dominant genes. Sex-Linked Disorders For sex-linked (also known as x-linked) recessive defective genes for which carrier tests exist, breeders should follow the same “breed and replace” recommendations as are outlined above in the discussion of autosomal recessive disorders. If there is no test, the defective gene can be traced through the pedigree. Selecting a normal male for breeding loses the defective gene in one generation, regardless of his relationship to affected and carrier relatives. Carrier, affected, or high risk females should not be used, due to the high risk of producing affected male offspring. If a male is affected, he would have received the defective gene from his carrier mother. All of his daughters will be carriers, but none of his sons. Without a test for carriers, you can use relative-risk assessment to breed him to a female that is at low risk of being a carrier. This minimizes the chance of producing affected offspring, and a quality son can be selected for replacement. Rare sex-linked dominant disorders are managed the same way as autosomal dominant disorders. The difference is that affected males will always produce all affected daughters. Polygenic disorders/Complex Inheritance Polygenic disorders are those caused by more than one pair of genes. A number of liability genes must combine to cross a threshold and produce an affected individual. Most polygenic disorders have no tests for carriers, but they do have phenotypic tests that can identify affected individuals. Controlling polygenically inherited disorders involves; 1) identifying traits that more closely represent genes being selected against, 2) the standardization of nuisance factors (such as environment) that can limit your selective pressure against the genes and 3) selecting for breadth of pedigree as well as depth of pedigree. In polygenic disorders, the phenotype of the individual does not directly represent its genotype. If phenotypically normal parents produce affected offspring, both should be considered to carry a genetic load of liability genes that combined to cause the disorder. Breeders must break down affected phenotypes into traits that more directly represent the genes that control them. For example, in hip dysplasia these can include clinical signs of lameness, shallow hip sockets, subluxation or remodeling on an extended leg view, and radiographic distractibility on a PennHIP view. If a quality individual is to be bred, but has shallow hip sockets, it should be bred to an individual with deep hip sockets. You need to select for enough genes influencing normal development, to get below the threshold where dysplasia develops. The environment has a role in the expression of polygenic disorders. Plane of nutrition and environmental stress, especially during critical growth periods can alter the expression of some inherited musculoskeletal disorders. You do not want to overly protect or overly stress the development of prospective breeding animals. Breeders should evaluate prospective breeding individuals raised under fairly uniform conditions, which will not mask or alter the expression of genetic disease. Polygenic disorders require knowledge of the affected or normal status of full-siblings to prospective breeding animals. Individuals whose siblings are normal and whose parents’ sibs are normal have the greatest chance of carrying a low genetic load for the condition. This breadth of pedigree analysis is more important than normalcy in the depth of pedigree (parents and grandparents only.) This is why it is important to screen both pet and breeding animals from litters for polygenic disorders, and report the results in open health registries, such as the not-for profit Orthopedic Foundation for Animals www.offa.org. Breadth of pedigree results can be visualized by clicking on vertical pedigrees on the individual dog’s OFA page. Affected individuals can be replaced with a normal sib or parent, and bred to a low-liability mate. Breeders can replace the higher risk parent with a quality, lower risk offspring, and repeat the process. In addition, the offspring of breeding dogs should be monitored to see which are passing the disorder with higher frequency. Undetermined Mode of Inheritance For disorders without a known mode of inheritance or carrier test, breeders should be counseled to use the same control methods as with polygenic disorders. Animals with a low genetic load for the disorder should be selected for breeding, through the results of examinations of first-degree relatives (littermates, parents, and offspring). If there are multiple generations of normalcy in the breadth of the pedigree, then you can have some confidence that there is less risk that liability genes are being carried. It is distressing when a genetic disorder is confirmed. Positive and practical genetic counseling recommendations can be made to maintain breed lines and genetic diversity, and improve the overall health of breeds. The total elimination of defective genes will probably be impossible for most breeds. The use of these guidelines can assist breeders in making objective breeding decisions for genetic disease management, while continuing their breeding lines. The individual breeder can use genetic tests to; 1) identify carriers, 2) work to breed away from the defective gene(s), and 3) ensure (through testing) that the defective gene(s) is not reintroduced in future matings. Each breeder will have their own rate of progress, depending on the frequency of the defective gene(s) in their own breeding animals, and which desirable individuals carry liability genes. |
